Methods and Reagents
The viral vector core (VVC) is located in the Whitehead Research Building 5th on the Emory University campus. The VVC is open to all Emory and non-Emory researchers. The mission of the Viral Vector Core is to pave the way for gene delivery. The Viral Vector Core is focused on high-titer, high-throughput production of Lentiviral vectors and recombinant adeno-associated viral vector (rAAV) to deliver and express interested genes in either dividing or non-dividing mammalian cells, primary culture andin vivo. The core has become an important technological resource for all investigators.
The specific objectives of the viral vector are to:
- Provide 2nd and 3rd generation lentiviral vector
- Provide rAAV serotypes 1, 2, 5, 6, 8, 9, PHP.eb and 2/rh10 vectors
- Provide lenti-CRISPR and AAV-CRISPR vectors
- Provide assistance in the design the viral vectors
Available Vectors
**Not all vector maps have been fully sequenced! The Viral Vector Core encourages all investigators to confirm their vector maps before initiating experimentation.
- LV 01- pFUGW
- LV 02- pCMO2
- LV 03- LV- CMV- GFP- Nhe
- LV 04- pLV- CMV- Cre
- LV 05- pFUW
- LV 06- sin- PGK- GFP- WH
- LV 07- pFUV5GW
- LV 08- pFCGW- N1 (PDF)
- LV 09- pFCYW- C1 (PDF)
- LV 10- pFCRW- N1 (PDF)
- LV 11- pFCCW- N1 (PDF)
- LV 12- pFH1W (PDF)
- LV 13- pFH1UGW (PDF)
- AAV01- pAAV-GFP (PDF)
- AAV02- pAAV-LacZ (PDF)
- AAV03- pAAV-MCS (PDF)
- AAV08- pAAV-CMV-CRE (PDF)
- Lenti-CRISPR
- AAV-CRISPR
- CRISPR library against human and mouse genome